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Vitamins & Hormones

Vitamin D Explained

More than a vitamin — a steroid hormone that regulates over 200 genes affecting immunity, mood, bones, and metabolic health.

Updated January 20269 min read

Who is this especially useful for?

  • People living in northern latitudes or with limited sun exposure
  • Those with darker skin tones (reduced vitamin D synthesis)
  • Individuals experiencing fatigue, mood issues, or frequent infections
  • Anyone on low-fat diets (vitamin D is fat-soluble)
  • People taking medications that deplete vitamin D (statins, corticosteroids, anticonvulsants)
  • Older adults (skin synthesis decreases with age)

Vitamin D deficiency is remarkably common — even in sunny climates due to indoor lifestyles and sun avoidance.

Vitamin D is often called a vitamin, but it's actually a steroid hormone that your body produces when sunlight hits your skin. This "sunshine hormone" affects virtually every cell in your body through vitamin D receptors (VDRs) found in tissues from your brain to your bones.

Despite its importance, vitamin D deficiency is epidemic — affecting an estimated 42% of US adults. Modern indoor lifestyles, sun avoidance, and living far from the equator have created widespread insufficiency that many don't recognize until symptoms appear.

How to Test Vitamin D Correctly

Not all vitamin D tests are equal. Here's what you need to know:

1
Best test: 25-hydroxyvitamin D (25-OH-D) — this is the storage form and reflects your true vitamin D status
2
Avoid: 1,25-dihydroxyvitamin D (1,25-OH-D) — this is the active form but doesn't reflect body stores; often normal even when deficient
3
Timing: Any time of year works, but late winter (Feb/Mar) shows your lowest baseline
4
Fasting: Not required for vitamin D testing
5
Retest interval: 3 months after changing supplementation to reach steady state; annually once stable
6
Cost: Often covered by insurance; approximately $50-80 if self-pay

💡 Pro tip: Test in February or March to see your lowest point of the year. This tells you if your winter sun exposure and supplementation are adequate.

Where to find your Vitamin D result

1
On your lab report: Look for "25-hydroxyvitamin D", "25-OH-D", or "Vitamin D, 25-Hydroxy"
2
Units matter: Results in ng/mL (US) or nmol/L (international). To convert: ng/mL × 2.5 = nmol/L
3
Ignore 1,25-OH-D: If you see "1,25-dihydroxyvitamin D", that's a different test — not what you need

Some labs report both forms. Focus only on 25-OH-D for assessing your vitamin D status.

What is Vitamin D?

Vitamin D is a fat-soluble secosteroid hormone essential for calcium absorption, bone health, immune function, and cellular processes. Unlike most vitamins, your body can produce it — when UVB rays from sunlight convert cholesterol in your skin to vitamin D3 (cholecalciferol).

Once produced or consumed, vitamin D undergoes two conversions: first in the liver to 25-hydroxyvitamin D (the storage form we measure), then in the kidneys and other tissues to 1,25-dihydroxyvitamin D (the active hormone that binds to vitamin D receptors).

Vitamin D receptors exist in over 30 tissues and regulate more than 200 genes — influencing everything from bone density to immune response to mood regulation. This is why deficiency can manifest in such diverse ways.

The Two Forms of Vitamin D

D3 (Cholecalciferol)

Produced by skin from sunlight; found in animal foods (fatty fish, egg yolks). More effective at raising blood levels.

D2 (Ergocalciferol)

Found in some mushrooms and fortified foods. Less potent and shorter-acting than D3.

Most experts recommend D3 for supplementation due to better efficacy and longer half-life.

42%

US Adults Deficient

Have levels below 20 ng/mL (NHANES data)

Forrest & Stuhldreher, 2011

200+

Genes Regulated

Vitamin D receptors affect gene expression throughout the body

Carlberg & Campbell, 2013

82%

COVID ICU Patients

Were vitamin D deficient in major study

Maghbooli et al., 2020

What Research Shows

Large-scale studies consistently show associations between low vitamin D and increased risk of respiratory infections, autoimmune diseases, cardiovascular events, and all-cause mortality. The 2017 Martineau meta-analysis of 25 RCTs found vitamin D supplementation reduced acute respiratory infections by 12% overall, with strongest effects in those starting with deficiency.

11,000+ participants across 25 randomized controlled trials

How Different Paradigms Interpret Vitamin D Levels

The definition of "optimal" vitamin D varies dramatically between medical approaches:

Standard Medical View

Thresholds: < 20 ng/mL deficient, 20-30 insufficient, 30-100 sufficient, > 100 potentially toxic

Ranges designed to prevent rickets, osteomalacia, and hypercalcemia. Values above 30 ng/mL considered adequate for bone health. Higher levels viewed with caution due to theoretical toxicity concerns.

Supplement only if deficient. Standard dose 600-800 IU daily per RDA. Retest annually if supplementing high doses.

Research Consensus View

Thresholds: < 30 deficient, 30-50 suboptimal, 50-80 optimal, 80-100 high normal, > 100 excess

Optimal range of 50-80 ng/mL associated with lowest all-cause mortality in epidemiological studies. Benefits extend beyond bones to immunity, cardiovascular health, cancer prevention, and mood. This is the range seen in outdoor workers and traditional populations.

Target 50-80 ng/mL through sensible sun exposure (15-30 min midday when possible) plus supplementation as needed. Many need 2,000-5,000 IU daily to reach and maintain optimal levels. Test twice yearly (late winter and late summer) initially.

Metabolic Optimization View

Thresholds: < 40 deficient, 40-60 suboptimal, 60-100 optimal, 100-150 high but generally safe, > 150 excess

Higher targets for metabolic and immune optimization. Vitamin D works synergistically with vitamin K2 (for calcium direction), magnesium (required for D metabolism), and adequate protein. Insulin sensitivity improves with higher D levels. Many ancestral health practitioners target the upper range.

Target 60-100 ng/mL. Supplement D3 (not D2) with K2-MK7 as co-factor — dosages vary widely in the metabolic health community, so consult your healthcare provider and consider testing (undercarboxylated osteocalcin) to personalize. Ensure adequate magnesium. Many in this community use 5,000-10,000 IU daily, adjusting seasonally. Monitor calcium if using high doses long-term.

Signs of Vitamin D Deficiency

Deficiency often develops slowly and symptoms are non-specific, making it easy to miss:

Fatigue and tiredness

Even with adequate sleep

Bone and back pain

Especially lower back

Depression and mood changes

Particularly in winter (SAD)

Impaired wound healing

Slower recovery from cuts, surgery

Bone loss

Low bone mineral density on DEXA

Hair loss

Especially alopecia areata

Muscle pain and weakness

Chronic aches without explanation

Frequent infections

Getting sick often, slow recovery

These symptoms have many causes. Testing is the only way to confirm vitamin D deficiency.

How to Optimize Your Vitamin D

Multiple strategies can help raise and maintain healthy vitamin D levels:

Sun Exposure

Midday sun is most efficient

10am-2pm when UVB rays are strongest

15-30 minutes on arms/legs

Without sunscreen for D synthesis

Skin tone matters

Darker skin needs 3-5x more exposure

UV index > 3 required

Below this, minimal D is produced

Dietary Sources

Fatty fish

Salmon, mackerel, sardines: 400-1000 IU/serving

Cod liver oil

1,400 IU per teaspoon (plus vitamin A)

Egg yolks (pastured)

40-50 IU each; more if hens had UV exposure

Beef liver

40-50 IU per serving

Supplementation

Choose D3 over D2

Cholecalciferol is more effective

Take with fat

Fat-soluble vitamin needs fat for absorption

Consider K2-MK7 co-factor

Helps direct calcium properly; dosing varies — consult provider

Don't forget magnesium

Required for vitamin D metabolism

Important Co-Factors

Vitamin D doesn't work in isolation. These nutrients affect its metabolism and function:

Vitamin K2 (MK-7)

Activates proteins that direct calcium to bones (not arteries). Critical at higher D doses.

Dosages discussed in metabolic health communities vary widely. Consider testing undercarboxylated osteocalcin to personalize. Consult your healthcare provider.

Magnesium

Required for converting vitamin D to its active form. Deficiency limits D effectiveness.

Many are deficient. Consider 200-400mg daily of well-absorbed forms (glycinate, malate).

Zinc

Needed for vitamin D receptor function and immune synergy.

15-30mg daily if deficient; balance with copper for long-term use.

Typical Improvement Timeline

Blood levels rise within 2-4 weeks of consistent supplementation. Steady state is reached at approximately 3 months. Symptoms like fatigue and mood often improve within 4-8 weeks of reaching optimal levels. Bone-related benefits take longer — 6-12 months for measurable changes in bone density.

What About Toxicity?

True vitamin D toxicity is rare and requires very high levels (typically > 150 ng/mL sustained) or extremely high supplementation (> 50,000 IU daily for extended periods). Symptoms include hypercalcemia (high blood calcium), nausea, weakness, and kidney issues. At reasonable supplementation levels with proper co-factors, toxicity is very unlikely. However, those taking more than 5,000 IU daily long-term should monitor levels periodically.

Key Takeaways

References

  1. Holick MF (2007). Vitamin D deficiency. N Engl J Med, 357(3), 266-281. PMID: 17634462
  2. Garland CF, et al (2007). Vitamin D and prevention of breast cancer: pooled analysis. J Steroid Biochem Mol Biol, 103(3-5), 708-711. PMID: 17368188
  3. Martineau AR, et al (2017). Vitamin D supplementation to prevent acute respiratory tract infections: systematic review and meta-analysis of individual participant data. BMJ, 356, i6583. PMID: 28202713
  4. Maghbooli Z, et al (2020). Vitamin D sufficiency, a serum 25-hydroxyvitamin D at least 30 ng/mL reduced risk for adverse clinical outcomes in patients with COVID-19 infection. PLoS One, 15(9), e0239799. PMID: 32976513
  5. Heaney RP (2008). Vitamin D in health and disease. Clin J Am Soc Nephrol, 3(5), 1535-1541. PMID: 18525006
  6. Cannell JJ, et al (2006). Epidemic influenza and vitamin D. Epidemiol Infect, 134(6), 1129-1140. PMID: 16959053
  7. Autier P, et al (2014). Vitamin D status and ill health: a systematic review. Lancet Diabetes Endocrinol, 2(1), 76-89. PMID: 24622671
  8. Pludowski P, et al (2018). Vitamin D supplementation guidelines. J Steroid Biochem Mol Biol, 175, 125-135. PMID: 28216084
  9. Wacker M, Holick MF (2013). Sunlight and Vitamin D: A global perspective for health. Dermatoendocrinol, 5(1), 51-108. PMID: 24494042
  10. Masterjohn C (2007). Vitamin D toxicity redefined: vitamin K and the molecular mechanism. Med Hypotheses, 68(5), 1026-1034. PMID: 17145139

Medical Disclaimer

This article is for educational purposes only and does not constitute medical advice. Vitamin D supplementation should be guided by testing and, ideally, in consultation with a healthcare provider — especially at higher doses or if you have kidney disease, sarcoidosis, or other conditions affecting calcium metabolism.